Evaluation of cell free circulating plasma DNA in prostate cancer

Document Type : Original Article

Authors

1 Women’s College for Arts, Science and Education, Ain Shams University.

2 National Cancer Institute, Cairo University,

3 Faculty of Medicine Kasr Al-Ainy, Cairo University.

Abstract

Background: Prostate cancer (PC) is the most common cancer affecting men, it accounts for 29% of all male cancer and 11% of all male cancer related deaths. DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell-free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe, which produce large fragments. Aim of work: Differentiate the cell free DNA levels (CFDNA) and its integrity in prostate cancer patients and control group composed of benign prostate hyperplasia (BPH) and healthy persons. Methodology: cf-DNA levels were quantified by real-time PCR amplification in prostate cancer patients (n = 50), (BPH) benign prostate hyperplasia (n = 25) and healthy controls (n = 30) using two sets of ALU gene (product size of 115 bp and 247-bp) and its integrity was calculated as a ratio of qPCR results of 247 bp ALU over 115 bp ALU. Results: Highly significant levels of cf-DNA and its integrity in PC patients compared to BPH. Twenty-eight (56%) patients with prostate cancer had bone metastasis. ALU115 q PCR is superior to the other markers in discriminating metastatic patients with a sensitivity of 96.4% and a specificity of 86.4% (AUC = 0.981) Conclusion: ALU115 q PCR could be used as a valuable biomarker helping in identifying high risk patients, indicating early spread of tumor cells as a potential seed for future metastases.
 

Keywords

Journal of Scientific Research in Science
Volume 34, part1
September 2017
Pages 108-122

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