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Journal of Scientific Research in Science
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Volume Volume 37 (2020)
Volume Volume 36 (2019)
Volume Volume 35 (2018)
Volume Volume 34 (2017)
Issue part1
Volume Volume 33 (2016)
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Khalil, M., Sabry, D., Mahdi, H. (2017). Green synthesis of silver, gold and silver-gold nanoparticles: Characterization, antimicrobial activity and cytotoxicity. Journal of Scientific Research in Science, 34(part1), 553-574. doi: 10.21608/jsrs.2018.14711
Mostafa M.H. Khalil; D. Y. Sabry; Huda Mahdi. "Green synthesis of silver, gold and silver-gold nanoparticles: Characterization, antimicrobial activity and cytotoxicity". Journal of Scientific Research in Science, 34, part1, 2017, 553-574. doi: 10.21608/jsrs.2018.14711
Khalil, M., Sabry, D., Mahdi, H. (2017). 'Green synthesis of silver, gold and silver-gold nanoparticles: Characterization, antimicrobial activity and cytotoxicity', Journal of Scientific Research in Science, 34(part1), pp. 553-574. doi: 10.21608/jsrs.2018.14711
Khalil, M., Sabry, D., Mahdi, H. Green synthesis of silver, gold and silver-gold nanoparticles: Characterization, antimicrobial activity and cytotoxicity. Journal of Scientific Research in Science, 2017; 34(part1): 553-574. doi: 10.21608/jsrs.2018.14711

Green synthesis of silver, gold and silver-gold nanoparticles: Characterization, antimicrobial activity and cytotoxicity

Article 38, Volume 34, part1, Summer 2017, Page 553-574  XML PDF (923.92 K)
Document Type: Original Article
DOI: 10.21608/jsrs.2018.14711
Authors
Mostafa M.H. Khalil; D. Y. Sabry; Huda Mahdi email
Chemistry Department, Faculty of Science, Ain Shams University, 11566, Abbassia, Cairo, Egypt
Abstract
The present study reported a facile and rapid biosynthesis method for gold nanoparticles (GNPs) silver nanoparticles (AgNPs) and bimetallic heterogeneous sliver-aurum nanoparticles(AgAuNPs) using the leaves of Gmelinaarborea (ROXB) (Family Verbenaceae) extract. The aqueous leaves extract was used as biotic reducing and stabilizing agent of the growing nanoparticles. The synthesized gold nanoparticles (AuNPs),silver nanoparticles (AgNPs) and silver-gold core-shell nanoparticles(AgAuNPs) were characterized using UV-Vis spectroscopy (UV–Vis), Fourier transform infrared spectroscopy, (FT-IR), X-ray diffraction (XRD),transmission electron microscopy (TEM)and thermal gravimetric analyses (TGA). Several factors such as the extract amount, contact time and solution pH, as possible influences; were investigated to obtain the optimized synthesis  conditions. The antimicrobial activity study revealed that while the aqueous extract at concentrations of0.8 and 4% (w/v) showed no effect on the antimicrobial activity, the produced nanoparticles, AuNPs, AgNPs and AgAuNPs inhibited thegrowth of Gram positive bacteria (Bacillus subtillus, Staphylococcus aureus), Gram negative bacteria (E. Coli and Pseudomonas aeruginosa) and Fungi (Candida albicansand Aspergillusniger). The cytotoxic activity against hepatocellular carcinoma (HePG2)was alsoevaluated.
 
 
Keywords
Biological synthesis; Gmelinaarborea; Gold nanoparticles; silver nanoparticles; silver-gold core-shell; nanoparticles, antibacterial activity; Hepatocellular carcinoma
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