Biodiesel production with high quality from Dunaleilla salina under optimization factors according to ASTM.

Document Type : Original Article

Authors

1 Department of Botany, Faculty of Science, Zagazig University, Zagazig.

2 Faculty of Women for Arts ,Science and Education-Ain Shams University

Abstract

MH medium was reprepared with the depriviation of nitrogen to 0.5g-1 KNO3 ,0.5g-1 MgCL2 and elevated the concentration of NaCL to 2.25M then, inoculated with Dunaliella salina in outdoor glass aquarium for three months. The culture was injected with 0.6 l-1co2 /day. The alga was semicontinuosly collected every four days. The collected samples were dried and used for biodiesel preparation. Results indicated that alga grown in modified medium shifted the biosynthesis of fatty acids towards the saturation level rather than unsaturated one. Since the relative percentages of saturated fatty acid (SFA) was 92.2% more than its corresponding control (basal medium) 65.6%. Analysis of  fatty acid profile obtained through gas liquid chromatography (GLC) revealed that the fatty acid methyl esters obtained from treated alga  (FAME) contains  palmitic acid (C16:0, 54%),myristic(C14:0, 23%) and stearic (C18:0, 3.2% ) with the high ratios rather than their corresponding ratios in control sample. Physical and chemical properties of biodiesel extracted from modified medium including cetane number, kinematics viscosity, flash point, pour point and cloud point are coping perfectly with ASTM. ASTM  is an international agency, known until 2001 as the American Society for Testing and Materials. Meaning that biodiesel of Dunaliella salina treated with modified mediumis a high quality biodiesel according to ASTM.
 

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