Albumin nanoparticles Preparation, Characterization and In-Vitro Safety Evaluation

Document Type : Original Article


1 Biotechnology Unit Animal Reproduction Research Institute (ARRi)

2 Faculty of Women for Arts, Science and Education, Ain Shams University, Egypt.

3 Nanotechnology and Advanced Material Central Lab, Agriculture Research Center, Egypt; Mostafa Elsayed Nanotechnology Research Centre, British University in Egypt, Egypt.

4 Biotechnology Research Unit, Animal Reproduction Research Institute, Egypt.

5 Immunology Unit, Animal Reproduction Research Institute, Egypt.

6 Nanotechnology Department, Agricultural Research Center, Giza, Egypt.


The goal of this study was to prepare and characterize albumin nanoparticles to be later used as a drug delivery system. The utilization of nanoparticles as a delivery system for antimicrobial drugs has arisen recently which solve many problems and enhance the traditional treatment with this antimicrobial drugs. In this study, nanoparticles of bovine serum albumen were successfully obtained using a coacervation process (separation of proteins in two liquid phases in colloidal systems).  The prepared nanoparticles were nearly spherical in shape and have smooth surface as determined by Transmission Electron Microscopy (TEM). The sizes of the obtained nanoparticles were 70 ± 10 nm with negative surface zeta potential. Additionally, the in vitro safety of albumin nanoparticles has been demonstrated. Both cytotoxicity and genotoxicity studies indicated that, there is no observed toxic effect of nano-albumin on lymphocyte cell line. Also, the results showed that the albumin nanoparticles enhances and promotes the response of immune system.