Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation

Hussein, M.; Hussein, M. M.; Kheiralla, Z. H.; Ahmed, H. A.; El-Gendy, N. Sh.

doi:10.21608/jsrs.2018.26739

Enhanced production of xylanase enzyme by Fusariummoniliforme using submerged fermentation

Document Type : Original Article

Authors

¹ Chemistry of Natural and Microbial Products Department, National Research Center, Dokki, Giza, Egypt.

² Botany Department,Faculty of Women for Arts, Science and Education, Ai Shams University.

³ Petroleum Biotechnology lab, Department of Processes Design & Development,Egyptian Petroleum Research Institute, Nasr City, Cairo, Egypt

10.21608/jsrs.2018.26739

Abstract

Hemicellulose degrading enzymes play an important role in bioconversion of agro and agro-industrial wastes. In this study, production of hemicellulase by six fungal isolates was determined under submerged culture using corn cobs xylan as a carbon source and enzyme inducer at different incubation periods. The results indicated that Alternariatenuisshowed the lowest enzyme productivity (156.95 ± 2.07U/l)while the highest enzyme production (2,594.44 ± 62.25U/l) was found by Fusariummoniliforme. One-factor-at-a-time (OFAT) revealed maximum enzyme productivity of 10,950.11 ± 98.45 U/l at; corn cobs xylan (6g/l), yeast extract (4g/l), inorganic salts (MgSO₄, KH₂PO₄, CaCl₂, FeSO₄, and MnSO₄), initial pH (5), initial inoculum size (4%), 150 rpm and temperature (30 ).

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