Morphological, cytogenetic and biochemical studies on the effects of zinc oxide in bulk form vs the nanoprepared form as fertilizers on Pisum sativum L. plant.

Document Type : Original Article

Authors

1 Botany Department, Faculty of Women For Arts, Science and Education Ain Shams University, Cairo, Egypt.

2 Genetics and Cytology Department, National Research Center, Dokki, Egypt.

3 Vegetable Crops Department, Faculty of Agriculture, Cairo University, Giza, Egypt.

4 Physiology Department, Faculty of Agriculture, Cairo University, Giza, Egypt.

Abstract

Zinc element is very essential in planting process; supplying plants with zinc element improve plant growth, development and performances in norm and under stresses. The new introduced nano zinc preparations should be evaluated and revised for their safety usage in agriculture. Methods: in this work the zinc oxide fertilizer in naturally bulk form is used as positive control to visualize effect of zinc oxide in nano form in different concentrations (50, 100 & 200ppm) on Pisum sativum plant. The study concerned some morphological traits and the total soluble seedling’s protein profile after the soil around root was injected with used materials, in addition to cytogenetic studies on the mitosis system after the direct treatment for 3hr and after the 24hr of recovery experiment. Results: the study confirms the great risk of the nanoforms usage on all the studied parameters on the level of the two higher concentrations if compared to -ve and +ve control. It induced lowering in all the morphological parameters. On the level of cytogenetic parameters, it empathized its chromo-toxic effect on the DNA physical constituent and mutagenic effect via induction of micro/ macronucleus in resting cells.  It also affected the gene expression via disappearance of some protein bands from image. Conclusion: lower concentration of nano zinc oxide particles can be used to enrich new reclaimed lands in limited conditions (if necessary) to avoid its mutagenic harm on plant as recommended alternative and to evade the risk of soil suffocation with  excess ZnO fertilizer in bulk form.

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